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작성일 : 18-08-01 10:32
Effector function of type II collagen-stimulated T cells from rheumatoid arthritis patients: cross-talk between T cells and synovial fibroblasts.
국내/국외 구분: 국외
SCI/SCIE/비SCI 구분: SCI
논문제목: Effector function of type II collagen-stimulated T cells from rheumatoid arthritis patients: cross-talk between T cells and synovial fibroblasts.
제 1저자, 교신저자: 김호연
나머지 저자: Cho ML, Yoon CH, Hwang SY, Park MK, Min SY, Lee SH, Park SH, Kim HY.
학술지명: Arthritis and Rheumatism.
Vol(No),페이지:
발행년월일: 2004/03/01
v파일다운로드: 2004/03/01

Abstract

OBJECTIVE:

To investigate the effector function exerted by type II collagen (CII)-stimulated T cells on rheumatoid arthritis (RA) fibroblast-like synoviocytes (FLS), and to determine their contribution to RA pathogenesis.

METHODS:

We used enzyme-linked immunosorbent assays to measure the levels of interleukin-15 (IL-15), tumor necrosis factor alpha (TNFalpha), and IL-18 production by FLS that were cocultured with antigen-activated T cells. Likewise, we analyzed the levels of interferon-gamma (IFN gamma) and IL-17 production by RA T cells coincubated with FLS. To investigate the cross-talk between CII-stimulated T cells and RA FLS, we examined the effect of using a transwell membrane to separate T cells and FLS in a culture chamber, as well as the effect of adding an antibody to block CD40 ligation.

RESULTS:

The levels of IL-15, TNF alpha, IFN gamma, and IL-17 were all significantly increased in the serum of RA patients compared with normal control serum. Among the patients, the group with a stronger T cell proliferation response to CII showed higher levels of these inflammatory mediators. When coincubated with RA FLS, these T cells induced the production of IL-15, TNF alpha, and IL-18 by FLS with an intensity that increased in proportion to the duration of CII stimulation. T cells, in turn, responded to FLS stimulation by secreting higher amounts of IL-17 and IFN gamma in coculture. Interestingly, T cells that were activated by CII for longer periods of time showed stronger induction of these cytokines. The cross-talk between T cells and FLS appeared to require direct cell-cell contact as well as CD40 ligation, at least in part.

CONCLUSION:

Through repeated stimulation by CII, RA synovial T cells became trained effector cells that induced the production of proinflammatory mediators by FLS, while in the process the T cells becoming more sensitized to the activation signal from FLS.


 
 

 
Total 180
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황수연, Cho ML, Kim JY, Ko HJ, Kim YH, Kim WU, Cho CS, Kim HY, Hwang SY.
The MCP-1 promoter -2518 polymorphism in Behcet's disease: correlation between allele types, MCP-1 production and clinical symptoms among Korean patients.
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김호연, Min DJ, Cho ML, Lee SH, Min SY, Kim WU, Min JK, Park SH, Cho CS, Kim HY.
Augmented production of chemokines by the interaction of type II collagen-reactive T cells with rheumatoid synovial fibroblasts.
Arthritis and Rheumatism., 2004/04/01,
국외
김호연, Cho ML, Yoon CH, Hwang SY, Park MK, Min SY, Lee SH, Park SH, Kim HY.
Effector function of type II collagen-stimulated T cells from rheumatoid arthritis patients: cross-talk between T cells and synovial fibroblasts.
Arthritis and Rheumatism., 2004/03/01,
국외
김호연, Min SY, Kim WU, Cho ML, Hwang SY, Park SH, Cho CS, Kim JM, Kim HY.
Prostaglandin E2 suppresses nuclear factor-kappaB mediated interleukin 15 production in rheumatoid synoviocytes.
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김호연, Cho ML, Kim WU, Min SY, Min DJ, Min JK, Lee SH, Park SH, Cho CS, Kim HY.
Cyclosporine differentially regulates interleukin-10, interleukin-15, and tumor necrosis factor a production by rheumatoid synoviocytes.
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김호연, Min DJ, Cho ML, Cho CS, Min SY, Kim WU, Yang SY, Min JK, Hong YS, Lee SH, Park SH, Kim HY.
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김호연, Park SH, Min DJ, Cho ML, Kim WU, Youn J, Park W, Cho CS, Kim HY.
Shift toward T helper 1 cytokines by type II collagen-reactive T cells in patients with rheumatoid arthritis.
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김호연, Cho CS, Cho ML, Min SY, Kim WU, Min DJ, Lee SS, Park SH, Choe J, Kim HY.
CD40 engagement on synovial fibroblast up-regulates production of vascular endothelial growth factor.
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김호연, Kim WU, Min SY, Cho ML, Youn J, Min DJ, Lee SH, Park SH, Cho CS, Kim HY.
The role of IL-12 in inflammatory activity of patients with rheumatoid arthritis.
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김호연, Kim HY, Kim WU, Cho ML, Lee SK, Youn JH, Kim SI, Yoo WH, Park JH, Min JK, Lee SH, Park SH, Cho CS.
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Arthritis and Rheumatism., 1999/10/01,
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, Jung SM, Lee J, Baek SY, Lee J, Jang SG, Hong SM, Park JS, Cho ML, Park SH, Kwok SK.
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, Lee SY, Min HK, Lee SH, Kwok SK, Ju JH, Cho ML, Park SH.
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Supplementary materials (Halofuginone ameliorates autoimmune arthritis by regulating the balance between Th17 and regulatory T cells and inhibiting osteoclastogenesis)
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